5C52
Probing the Structural and Molecular Basis of Nucleotide Selectivity by Human Mitochondrial DNA Polymerase gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 217.277, 217.277, 165.865 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.213 - 3.637 |
R-factor | 0.3112 |
Rwork | 0.311 |
R-free | 0.31370 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.826 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.213 |
High resolution limit [Å] | 3.560 |
Number of reflections | 47896 |
<I/σ(I)> | 2 |
Completeness [%] | 97.0 |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | PEG4000, KCl, HEPES |