5C4Y

Crystal structure of putative TetR family transcription factor from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	195.697, 52.169, 50.403
Unit cell angles	90.00, 104.97, 90.00

Refinement procedure

Resolution	33.838 - 1.770
R-factor	0.1643
Rwork	0.162
R-free	0.20620
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.578
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX ((dev_2056: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.770	4.800	1.770
Rmerge	0.054	0.022	0.422
Total number of observations	297155
Number of reflections	47069
<I/σ(I)>	11.6
Completeness [%]	98.6	97.7	87.5
Redundancy	6.3	6.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	12% PEG6000, 2.0M sodium chlorode