5C4W
Crystal structure of coxsackievirus A16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 294 |
| Detector technology | PIXEL |
| Collection date | 2012-09-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 491.200, 491.200, 708.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.870 - 2.650 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vbf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.519 | |
| Number of reflections | 1705020 | |
| <I/σ(I)> | 1.5 | 0.4 |
| Completeness [%] | 69.6 | 31.8 |
| Redundancy | 2.1 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate |
