5C4N
CobK precorrin-6A reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.870, 73.140, 103.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.170 - 1.630 |
R-factor | 0.17632 |
Rwork | 0.175 |
R-free | 0.20820 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.303 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.170 | 1.720 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 32962 | |
<I/σ(I)> | 19.4 | 4.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100 |