5C4M

RhoA GDP with novel switch II conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	31.796, 66.271, 83.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.300 - 1.300
R-factor	0.17147
Rwork	0.170
R-free	0.19953
RMSD bond length	0.007
RMSD bond angle	1.343
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.300
High resolution limit [Å]	1.300
Number of reflections	44107
<I/σ(I)>	20.3
Completeness [%]	99.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% (w/v) PEG 3350, 0.3 NaCl