5C4J
Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2006-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 280.711, 223.384, 156.422 |
| Unit cell angles | 90.00, 98.14, 90.00 |
Refinement procedure
| Resolution | 174.080 - 4.000 |
| R-factor | 0.21438 |
| Rwork | 0.212 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.921 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 174.080 | 4.104 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.147 | 0.610 |
| Number of reflections | 80485 | |
| <I/σ(I)> | 10.3 | 3.7 |
| Completeness [%] | 97.0 | 96.1 |
| Redundancy | 5.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | PEG 4000, SODIUM MALONATE, HEPES, DTT |
