5C41
Crystal structure of human ribokinase in complex with AMPPCP in P21 spacegroup and with 4 protomers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.950, 89.190, 144.400 |
| Unit cell angles | 90.00, 98.16, 90.00 |
Refinement procedure
| Resolution | 44.550 - 1.950 |
| R-factor | 0.17394 |
| Rwork | 0.172 |
| R-free | 0.20733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.011 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.550 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.096 | 0.727 |
| Number of reflections | 94826 | |
| <I/σ(I)> | 11.1 | 1.9 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M sodium HEPES, 10% PEG 8000 |
