5C3Y
Structure of human ribokinase crystallized with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.310, 168.140, 161.500 |
| Unit cell angles | 90.00, 90.82, 90.00 |
Refinement procedure
| Resolution | 51.260 - 2.600 |
| R-factor | 0.20609 |
| Rwork | 0.205 |
| R-free | 0.23205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.903 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.260 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.135 | 0.703 |
| Number of reflections | 130659 | |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 0.1M sodium HEPES, 7% PEG 8000 |
