5C3X
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-01-16 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.126, 60.173, 38.047 |
Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
Resolution | 37.900 - 1.950 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.877 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.020 | 0.119 |
Total number of observations | 247526 | |
Number of reflections | 10271 | |
<I/σ(I)> | 36.06 | 7.89 |
Completeness [%] | 100.0 | 100 |
Redundancy | 23.95 | 17.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |