5C3M
Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.324, 83.198, 151.372 |
| Unit cell angles | 90.00, 113.61, 90.00 |
Refinement procedure
| Resolution | 46.603 - 3.059 |
| R-factor | 0.2334 |
| Rwork | 0.230 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s6y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.100 | 0.561 |
| Number of reflections | 39747 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 99.4 | 94.2 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 16% PEG 8K, 0.1M CHES buffer pH 9.5 |
