5C3L

Structure of the metazoan Nup62.Nup58.Nup54 nucleoporin complex.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97864
Spacegroup name	P 65 2 2
Unit cell lengths	167.570, 167.570, 142.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.878 - 2.900
R-factor	0.2658
Rwork	0.265
R-free	0.30070
Structure solution method	SAD
Starting model (for MR)	4hep
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.000		2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.235	0.040	6.106
Rmeas	0.236	0.040	6.123
Total number of observations	5534630
Number of reflections	49303	404	2058
<I/σ(I)>	39.44	152.14	1.66
Completeness [%]	98.8	97.6	96.3
Redundancy	101

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	PEG MME 2000, Trimethylamine N-oxide, Tris-HCl