5C3E

Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	219.050, 390.940, 278.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.700
R-factor	0.2012
Rwork	0.201
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fki
RMSD bond length	0.008
RMSD bond angle	1.090
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	BUSTER-TNT (BUSTER 2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	195.470	90.363	3.580
High resolution limit [Å]	3.400	10.750	3.400
Rmerge		0.122	1.413
Rmeas	0.373
Rpim	0.189	0.072	0.854
Total number of observations	515843	19594	42222
Number of reflections	145314
<I/σ(I)>	3.2	6.7	0.9
Completeness [%]	89.3	99.3	55.4
Redundancy	3.5	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	PEG 6000, Ammonium Acetate, Sodium Acetate, DTT