5C3C
Structural characterization of a newly identified component of alpha-carboxysomes: The AAA+ domain Protein cso-CbbQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00003 |
| Spacegroup name | H 3 |
| Unit cell lengths | 171.913, 171.913, 53.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.798 - 2.800 |
| R-factor | 0.2313 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.113 | 0.314 |
| Number of reflections | 14510 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 5 mg/ml protein, 0.1 M HEPES, 8 % ethylene glycol, 6% PEG-8000 |
