5C35
Constitutively active Sin recombinase cataltyic domain - T77II100T/Q115R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97907 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.842, 73.842, 180.483 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.172 - 2.400 |
R-factor | 0.3179 |
Rwork | 0.316 |
R-free | 0.33530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pkz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((phenix.refine: dev_1951)) |
Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.400 | 2.400 |
Rmerge | 0.101 | 0.073 | 0.000 |
Rmeas | 0.106 | 0.076 | |
Rpim | 0.028 | 0.019 | |
Total number of observations | 211480 | ||
Number of reflections | 12607 | ||
<I/σ(I)> | 35.8 | 0.4 | |
Completeness [%] | 99.7 | 98.7 | 96.5 |
Redundancy | 17.3 | 15.9 | 6.5 |
CC(1/2) | 0.998 | 0.189 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 10mM DTT, 500mM HEPES, pH 7.0, 0.96 M AmSO4, 8% PEG 400 |