5C2O

Crystal structure of Streptococcus mutans Deoxycytidylate Deaminase complexed with dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.2824
Spacegroup name	P 21 3
Unit cell lengths	112.455, 112.455, 112.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.189 - 2.350
R-factor	0.1846
Rwork	0.183
R-free	0.21160
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	19994
<I/σ(I)>	56.2
Completeness [%]	100.0
Redundancy	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG3350