5C2I
Crystal structure of Anabaena sp. DyP-type peroxidese (AnaPX)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.734, 132.601, 176.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.730 - 1.890 |
| R-factor | 0.1817 |
| Rwork | 0.181 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g2c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.453 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.1.03) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | |
| Number of reflections | 207238 | |
| <I/σ(I)> | 17.62 | |
| Completeness [%] | 90.5 | 81.2 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG 4000 |
