5C2D

K428A mutant gp2c of large terminase subunit from bacteriophage sf6 with calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.03361
Spacegroup name	C 1 2 1
Unit cell lengths	133.078, 57.437, 46.568
Unit cell angles	90.00, 98.99, 90.00

Refinement procedure

Resolution	18.946 - 1.590
R-factor	0.1613
Rwork	0.160
R-free	0.19150
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	1.620
High resolution limit [Å]	1.590	1.590
Rmerge	0.052	0.814
Number of reflections	46066
<I/σ(I)>	16.1	1.2
Completeness [%]	98.6	90.7
Redundancy	3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000