5C1T
Crystal structure of the GTP-bound wild type EhRabX3 from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 |
Unit cell lengths | 62.965, 62.965, 312.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.692 - 2.801 |
R-factor | 0.2665 |
Rwork | 0.263 |
R-free | 0.30210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.692 |
High resolution limit [Å] | 2.800 |
Number of reflections | 16942 |
<I/σ(I)> | 12.5 |
Completeness [%] | 98.9 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 200mM Ammonium sulphate, 100mM BisTris, 25% PEG 3350 |