5C19
p97 variant 2 in the apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 137.665, 165.780, 243.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.129 - 4.200 |
R-factor | 0.2402 |
Rwork | 0.237 |
R-free | 0.30230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cf3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.597 |
Data scaling software | Aimless (0.3.8) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.130 | 49.130 | 4.370 |
High resolution limit [Å] | 4.200 | 15.140 | 4.200 |
Rmerge | 0.259 | 0.044 | 1.197 |
Rpim | 0.107 | 0.021 | 0.484 |
Total number of observations | 277534 | 5075 | 32400 |
Number of reflections | 41484 | ||
<I/σ(I)> | 7.3 | 30.6 | 1.8 |
Completeness [%] | 99.9 | 96.3 | 100 |
Redundancy | 6.7 | 5.3 | 7 |
CC(1/2) | 0.992 | 0.997 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% PEG 4000, 0.15 M ammonium sulfate, 0.1 M MES pH 6.5 |