5C10
Nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-08-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.03316 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.683, 57.734, 46.654 |
Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
Resolution | 19.044 - 1.550 |
R-factor | 0.1455 |
Rwork | 0.144 |
R-free | 0.16470 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.690 | 29.690 | 1.570 |
High resolution limit [Å] | 1.550 | 8.470 | 1.550 |
Rmerge | 0.040 | 0.033 | 0.608 |
Rpim | 0.025 | 0.024 | 0.370 |
Total number of observations | 184520 | 996 | 8045 |
Number of reflections | 50109 | ||
<I/σ(I)> | 17.7 | 48.7 | 1.9 |
Completeness [%] | 97.7 | 91.9 | 90.8 |
Redundancy | 3.7 | 3.2 | 3.5 |
CC(1/2) | 0.999 | 0.994 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000 |