5C0F
HLA-A02 carrying RQWGPDPAAV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.360, 79.780, 56.970 |
| Unit cell angles | 90.00, 115.70, 90.00 |
Refinement procedure
| Resolution | 42.840 - 1.463 |
| R-factor | 0.1536 |
| Rwork | 0.152 |
| R-free | 0.18080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3utq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.086 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.8.0023) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.841 | 42.841 | 1.500 |
| High resolution limit [Å] | 1.460 | 6.540 | 1.460 |
| Rmerge | 0.020 | 0.545 | |
| Rmeas | 0.069 | ||
| Rpim | 0.034 | 0.014 | 0.362 |
| Total number of observations | 313592 | 3473 | 23295 |
| Number of reflections | 77814 | ||
| <I/σ(I)> | 14.7 | 44.4 | 2.3 |
| Completeness [%] | 99.4 | 98.3 | 100 |
| Redundancy | 4 | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M TRIS, pH 8, 20% PEG 8000, 15% glycerol |
