5BZY
X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77.15 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 25.958, 25.958, 121.533 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.510 - 2.400 |
| R-factor | 0.25046 |
| Rwork | 0.245 |
| R-free | 0.31275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bxw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.480 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.093 | 0.208 |
| Number of reflections | 2131 | |
| <I/σ(I)> | 8.9 | 2.6 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD |
