5BZW
Crystal Structure of PCDN-27B, an antibody from the PCDN family of HIV-1 antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 273.15 |
| Detector technology | PIXEL |
| Collection date | 2015-05-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.354, 89.354, 211.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.629 - 2.900 |
| R-factor | 0.2364 |
| Rwork | 0.233 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.629 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.993 | |
| Number of reflections | 11743 | |
| <I/σ(I)> | 9.8 | 2.8 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 13.9 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% (w/v) PEG 3350, 10% (v/v) ethylene glycol and 0.2M ammonium sulfate |
