5BZV
Crystal structure of the RNA-binding domain of yeast Puf5p bound to SMX2 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 106.930, 106.930, 167.352 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.053 - 2.354 |
R-factor | 0.1923 |
Rwork | 0.188 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bym |
RMSD bond length | 0.007 |
RMSD bond angle | 0.978 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.098 | 0.404 |
Number of reflections | 22619 | |
<I/σ(I)> | 22.9 | 3.8 |
Completeness [%] | 93.0 | 83.2 |
Redundancy | 7.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 15-20% PEG3350, 0.1M CBTP, pH 7.6 |