5BZ9
X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77.15 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979200 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 25.989, 25.989, 121.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.510 - 2.100 |
R-factor | 0.27493 |
Rwork | 0.271 |
R-free | 0.31152 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bxw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.406 |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.510 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.138 | 0.244 |
Number of reflections | 3035 | |
<I/σ(I)> | 16.3 | 3 |
Completeness [%] | 97.8 | 97.4 |
Redundancy | 10.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD |