5BZ5

Crystal structure of the RNA-binding domain of yeast Puf5p bound to AMN1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	105.852, 105.852, 168.404
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.509 - 2.800
R-factor	0.2071
Rwork	0.201
R-free	0.25850
RMSD bond length	0.003
RMSD bond angle	0.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.094	0.568
Number of reflections	13482
<I/σ(I)>	20	2
Completeness [%]	93.7	91.7
Redundancy	6.9	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	PEG3350, CBTP