5BZ3
CRYSTAL STRUCTURE OF SODIUM PROTON ANTIPORTER NAPA IN OUTWARD-FACING CONFORMATION.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.580, 94.090, 147.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 2.300 |
R-factor | 0.2065 |
Rwork | 0.206 |
R-free | 0.21850 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.958 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1938) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.182 | 1.036 |
Number of reflections | 17750 | |
<I/σ(I)> | 12.24 | 1.65 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.5 | 4.5 |
CC(1/2) | 0.972 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.4 | 293 | Lipid: MAG 7.7 (Lot: 141MG(7.7)-15, Avanti) protein (30mg/ml) weight ratio 1:1 |