5BYF
Crystal structure of human ribokinase in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.090, 63.870, 181.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.500 - 2.000 |
| R-factor | 0.17704 |
| Rwork | 0.175 |
| R-free | 0.22186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.124 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.905 |
| Number of reflections | 44741 | |
| <I/σ(I)> | 15.1 | 2.1 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris, 8% PEG 8000 |
