5BYE
Crystal structure of human ribokinase in P212121 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.250, 65.480, 180.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.740 - 1.750 |
| R-factor | 0.19221 |
| Rwork | 0.190 |
| R-free | 0.22882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.275 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.086 | 0.962 |
| Number of reflections | 67798 | |
| <I/σ(I)> | 16.2 | 2.3 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 10.6 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris, 8% PEG 8000 |
