5BYC
Crystal structure of human ribokinase in C2 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.920, 47.040, 80.910 |
| Unit cell angles | 90.00, 98.73, 90.00 |
Refinement procedure
| Resolution | 45.490 - 1.950 |
| R-factor | 0.17507 |
| Rwork | 0.173 |
| R-free | 0.22044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.112 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.132 | 1.002 |
| Number of reflections | 49417 | |
| <I/σ(I)> | 11.5 | 1.9 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M sodium HEPES, 10% isopropanol, 20% PEG 4000, 0.1M ammonium sulfate |
