5BY3
A novel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795, 0.9798, 0.9682 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.770, 102.300, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.440 |
R-factor | 0.19326 |
Rwork | 0.189 |
R-free | 0.26583 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.848 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.510 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.614 | |
Number of reflections | 32214 | |
<I/σ(I)> | 17.6 | 2.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 291 | 20% PEG 3350 0.2M sodium sulfate 0.01M glycine |