5BY0
Crystal structure of magnesium-bound Duf89 protein Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.407, 108.582, 88.234 |
| Unit cell angles | 90.00, 97.04, 90.00 |
Refinement procedure
| Resolution | 34.400 - 1.800 |
| R-factor | 0.1593 |
| Rwork | 0.157 |
| R-free | 0.19480 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | 0.660 |
| Number of reflections | 46859 | |
| <I/σ(I)> | 28 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | 0.1 M MgCl2, 0.1 M Bis-Tris, 25% Peg3350 |
