5BXZ

H17 Bat Influenza NS1 RNA Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	46.675, 46.675, 60.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.337 - 2.600
R-factor	0.1837
Rwork	0.173
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.640
High resolution limit [Å]	2.600	7.030	2.600
Rmerge	0.131	0.063	0.432
Total number of observations	28904
Number of reflections	4036
<I/σ(I)>	9.2
Completeness [%]	100.0	99.1	100
Redundancy	7.2	6.7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	292	0.1M HEPES pH 7.5, 25% PEG 1000