5BXW
X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77.15 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979200 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 25.827, 25.827, 123.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.030 - 2.150 |
| R-factor | 0.24624 |
| Rwork | 0.244 |
| R-free | 0.26275 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.887 |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.030 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.149 | 0.282 |
| Number of reflections | 2965 | |
| <I/σ(I)> | 14.7 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD |
