5BXJ
Complex of the Fk1 domain mutant A19T of FKBP51 with 4-Nitrophenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.127, 53.720, 56.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.024 - 1.240 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.20560 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.303 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.240 |
Number of reflections | 36610 |
<I/σ(I)> | 23.8 |
Completeness [%] | 99.2 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 30% PEG 3350, 0.2 M Ammonium Acetate and 0.1 M HEPES pH 7.5 |