5BXH
Crystal structure of pentameric KCTD9 BTB domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.396, 105.396, 97.316 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.566 - 2.760 |
R-factor | 0.2367 |
Rwork | 0.234 |
R-free | 0.27990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3drz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.570 | 2.858 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.057 | 0.829 |
Number of reflections | 16374 | |
<I/σ(I)> | 10.09 | 2.46 |
Completeness [%] | 99.7 | 99.63 |
Redundancy | 9.8 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 25 mg/mL KCTD9 BTB domain with: 0.17 M Lithium citrate, 16% w/v PEG 3350. 1:1 ratio |