5BXD
Crystal structure of pentameric KCTD1 BTB domain form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.064, 90.839, 127.694 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.080 - 1.796 |
R-factor | 0.2036 |
Rwork | 0.203 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3drz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.829 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.080 | 1.860 |
High resolution limit [Å] | 1.796 | 1.796 |
Rmerge | 0.022 | 0.300 |
Number of reflections | 50809 | |
<I/σ(I)> | 16.98 | 2.37 |
Completeness [%] | 99.8 | 99.52 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25 mg/mL KCTD1 BTB domain with: 0.1 M MES pH 5, 10% w/v PEG 6000. 1:1 ratio |