5BXB
Crystal structure of pentameric KCTD1 BTB domain form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.885, 83.055, 94.763 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 94.760 - 2.171 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.760 | 2.249 |
| High resolution limit [Å] | 2.171 | 2.171 |
| Rmerge | 0.037 | 0.395 |
| Number of reflections | 54500 | |
| <I/σ(I)> | 12.37 | 2.23 |
| Completeness [%] | 99.2 | 99.76 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 10 mg/mL KCTD1 BTB domain with: 0.1 M MES pH 6, 5% w/v PEG 1000, 30% w/v PEG 600, 10% v/v glycerol. 1:1 ratio |
