5BWA

Crystal structure of ODC-PLP-AZ1 ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-13
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	53.154, 74.084, 157.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.759 - 3.200
R-factor	0.232
Rwork	0.229
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d7k 1zo0
RMSD bond length	0.006
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.540	3.270
High resolution limit [Å]	3.200	3.200
Rmerge	0.084	0.667
Number of reflections	10195
<I/σ(I)>	15.7	3.4
Completeness [%]	94.7	94.4
Redundancy	10	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	288	0.2 M di-Ammonium tartrate, 20% PEG 3350