5BVA

Structure of flavin-dependent brominase Bmp2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	82.975, 90.481, 57.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.228 - 1.873
R-factor	0.19
Rwork	0.188
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e1t
RMSD bond length	0.007
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.870
Rmerge	0.082
Number of reflections	36287
<I/σ(I)>	24.93
Completeness [%]	100.0
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG