5BV3

Yeast Scavenger Decapping Enzyme in complex with m7GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	87.990, 104.520, 189.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.250
R-factor	0.2228
Rwork	0.222
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xmm
RMSD bond length	0.010
RMSD bond angle	1.354
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.079	0.031	0.716
Rmeas	0.089	0.035	0.851
Total number of observations	361983
Number of reflections	83079	849	6099
<I/σ(I)>	12.57	37.6	2.32
Completeness [%]	99.1	79.4	99.5
Redundancy	2.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100 mM Hepes, 100 mM NaCl, 1.6 M AmSO4