5BV1

Crystal Structure of a Vps33-Vps16 Complex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 1 21 1
Unit cell lengths	79.280, 159.902, 97.394
Unit cell angles	90.00, 90.21, 90.00

Refinement procedure

Resolution	61.592 - 2.902
R-factor	0.2148
Rwork	0.211
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmo
RMSD bond length	0.006
RMSD bond angle	1.056
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	159.902	159.902	2.912
High resolution limit [Å]	2.902	13.469	2.902
Rmerge	0.085	0.024	0.807
Total number of observations	300496	2923	3187
Number of reflections	53085
<I/σ(I)>	16.9		2.1
Completeness [%]	99.0	97.3	98
Redundancy	5.7	5.3	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	HEPES buffer, 8-10% w/v PEG 5000 monomethyl ether