5BUZ

Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 1 21 1
Unit cell lengths	95.039, 95.950, 164.060
Unit cell angles	90.00, 99.12, 90.00

Refinement procedure

Resolution	47.975 - 3.100
R-factor	0.1955
Rwork	0.194
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmo
RMSD bond length	0.003
RMSD bond angle	0.797
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.102	0.041	0.861
Total number of observations	199852
Number of reflections	54752
<I/σ(I)>	6.3
Completeness [%]	100.0	99.8	99.9
Redundancy	3.7	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	HEPES buffer, 8-10% w/v PEG 5000 monomethyl ether