5BUZ
Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.039, 95.950, 164.060 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 47.975 - 3.100 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kmo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.797 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.102 | 0.041 | 0.861 |
| Total number of observations | 199852 | ||
| Number of reflections | 54752 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 100.0 | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | HEPES buffer, 8-10% w/v PEG 5000 monomethyl ether |
