5BUY
Crystal Structure of beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.709, 56.029, 140.089 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.550 |
| R-factor | 0.21297 |
| Rwork | 0.209 |
| R-free | 0.28117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zhg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.085 | 0.224 |
| Number of reflections | 31876 | |
| <I/σ(I)> | 18.8 | 1.5 |
| Completeness [%] | 90.9 | 90.9 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350 |
