5BUN
Crystal structure of an antigenic outer membrane protein ST50 from Salmonella Typhi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-25 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | I 4 |
Unit cell lengths | 246.847, 246.847, 65.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.935 - 2.980 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.27510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ek9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.931 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.980 |
Rmerge | 0.110 |
Number of reflections | 40826 |
<I/σ(I)> | 10.5 |
Completeness [%] | 99.8 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 10% PEG 8000, 100mM NaCl, 0.5% |