5BUF

2.37 Angstrom Structure of EPSP Synthase from acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-18
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	73.893, 103.380, 113.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.791 - 2.370
R-factor	0.1856
Rwork	0.183
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o0b
RMSD bond length	0.004
RMSD bond angle	0.798
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1682))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.791	2.410
High resolution limit [Å]	2.370	2.370
Rmerge		0.420
Number of reflections	34793
<I/σ(I)>	17.5	4.74
Completeness [%]	96.9	99
Redundancy	7.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	100 mM Bis-Tris propane, pH 7.0, 100 mM potassium bromide, 40% (w/v) PEG 8000