5BU4
RIBONUCLEASE T1 COMPLEX WITH 2'GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.470, 48.100, 50.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.770 |
R-factor | 0.172 * |
Rwork | 0.172 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7gsp |
RMSD bond length | 0.013 * |
RMSD bond angle | 2.354 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | RESTRAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.770 | |
Rmerge | 0.188 * | 0.978 * |
Total number of observations | 380597 * | |
Number of reflections | 9833 | |
<I/σ(I)> | 23.64 | |
Completeness [%] | 93.8 | |
Redundancy | 38.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.2 | Zegers, I., (1998) Nat. Struct. Biol., 5, 280. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | 2'GMP | 2 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | 2 (mM) | ||
5 | 1 | reservoir | MPD | 55 (%(v/v)) |