5BU2
Structure of the C-terminal domain of lpg1496 from Legionella pneumophila in complex with nucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 1 |
Unit cell lengths | 56.867, 69.707, 77.027 |
Unit cell angles | 89.87, 72.30, 70.31 |
Refinement procedure
Resolution | 34.000 - 2.110 |
R-factor | 0.23123 |
Rwork | 0.229 |
R-free | 0.27307 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bu1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.212 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.435 | |
Number of reflections | 54209 | |
<I/σ(I)> | 11.3 | 1.7 |
Completeness [%] | 95.0 | 78.3 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 25% (w/v) PEG3350, 5 mM ADP |