5BTZ
Structure of the middle domain of lpg1496 from Legionella pneumophila in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-31 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.137, 44.066, 77.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.780 - 1.600 |
R-factor | 0.20124 |
Rwork | 0.200 |
R-free | 0.23125 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.406 | |
Number of reflections | 16100 | |
<I/σ(I)> | 49.7 | 4.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.6 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 25% (w/v) PEG3350 |