5BTW
Structure of the N-terminal domain of lpg1496 from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.410, 56.920, 60.184 |
Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.1586 |
Rwork | 0.158 |
R-free | 0.17952 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bty |
RMSD bond length | 0.012 |
RMSD bond angle | 1.462 |
Data reduction software | HKL-2000 (5.8.0049) |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.567 | |
Number of reflections | 60366 | |
<I/σ(I)> | 43.9 | 2.8 |
Completeness [%] | 92.7 | 84.3 |
Redundancy | 7.7 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium formate, 20% (w/v) PEG3350 |