5BTW
Structure of the N-terminal domain of lpg1496 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.410, 56.920, 60.184 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.200 |
| R-factor | 0.1586 |
| Rwork | 0.158 |
| R-free | 0.17952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bty |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-2000 (5.8.0049) |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.567 | |
| Number of reflections | 60366 | |
| <I/σ(I)> | 43.9 | 2.8 |
| Completeness [%] | 92.7 | 84.3 |
| Redundancy | 7.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium formate, 20% (w/v) PEG3350 |
